From pre-analytical QC to running the sequencer, this is the place to find the latest tips and advice from
our sequencing experts for all stages of the NGS protocol.
The latest tip for NGS Success!
Captured DNA remains on the beads and will proceed into the Post-Capture PCR. DO NOT discard the beads during the final wash steps, as you would lose your enriched DNA. The Streptavidin beads do not affect the efficiency of the post-capture PCR.
View our collection of NGS tips for...
- Pre-analytical QC
- All Purification Procedures
- DNA Shearing
- End repair and 3’ end ‘A’ Tailing
- Adaptor ligation
- PCR1 Optimisation
- Hybridisation Temperature Optimisation
- Preparing Magnetic Beads
- Hybrid Capture
- Washing Streptavidin Beads
- Post-Capture PCR
- Running the sequencer
SureSeq™: For research use only; not for use in diagnostic procedures.